التفاصيل البيبلوغرافية
| العنوان: |
Oxidized low-density lipoprotein and β-glycerophosphate synergistically induce endothelial progenitor cell ossification. |
| المؤلفون: |
Liu, Li, Liu, Zhi-zhong, Chen, Hui, Zhang, Guo-jun, Kong, Yu-hua, Kang, Xi-xiong |
| المصدر: |
Acta Pharmacologica Sinica; Dec2011, Vol. 32 Issue 12, p1491-1497, 7p |
| مصطلحات موضوعية: |
LOW density lipoproteins, ENDOTHELIUM, OXIDATIVE stress, REVERSE transcriptase polymerase chain reaction, GENE expression, LABORATORY rats |
| مستخلص: |
Aim:To investigate the ability of ox-LDL to induce ossification of endothelial progenitor cells (EPCs) in vitro and explored whether oxidative stress, especially hypoxia inducible factor-1α (HIF-1α) and reactive oxygen species (ROS), participate in the ossific process.Methods:Rat bone marrow-derived endothelial progenitor cells (BMEPCs) were cultured in endothelial growth medium supplemented with VEGF (40 ng/mL) and bFGF (10 ng/mL). The cells were treated with oxidized low-density lipoprotein (ox-LDL, 5 μg/mL) and/or β-glycerophosphate (β-GP, 10 mmol/L). Calcium content and Von Kossa staining were used as the measures of calcium deposition. Ossific gene expression was determined using RT-PCR. The expression of osteocalcin (OCN) was detected with immunofluorescence. Alkaline phosphatase (ALP) activity was analyzed using colorimetric assay. Intercellular reactive oxygen species (ROS) were measured with flow cytometry.Results:BMEPCs exhibited a spindle-like shape. The percentage of cells that expressed the cell markers of EPCs CD34, CD133 and kinase insert domain-containing receptor (KDR) were 46.2%±5.8%, 23.5%±4.0% and 74.3%±8.8%, respectively. Among the total cells, 78.3%±4.2% were stained with endothelial-specific fluorescence. Treatment of BMEPCs with ox-LDL significantly promoted calcium deposition, which was further significantly enhanced by co-treatment with β-GP. The same treatments significantly increased the gene expression of core-binding factor a-1 (cbfa-1) and OCN, while decreased the gene expression of osteoprotegerin (OPG). The treatments also significantly enhanced the activity of ALP, but did not affect the number of OCN+ cells. Furthermore, the treatments significantly increased ROS and activated the hypoxia inducible factor-1α (HIF-1α). In all these effects, ox-LDL acted synergistically with β-GP.Conclusion:Ox-LDL and β-GP synergistically induce ossification of BMEPCs, in which an oxidizing mechanism is involved. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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