دورية أكاديمية

Pulsed-field gel electrophoresis study of the genome of Caldocellum saccharolyticum.

التفاصيل البيبلوغرافية
العنوان: Pulsed-field gel electrophoresis study of the genome of Caldocellum saccharolyticum.
المؤلفون: Borges, Kim, Bergquist, Peter
المصدر: Current Microbiology; Jul1993, Vol. 27 Issue 1, p15-19, 5p
مستخلص: The genome of the anaerobic thermophilic eubacterium Caldocellum saccharolyticum was analyzed by pulsed field gel electrophoresis. The restriction endonucleases NheI and SstII cleaved the genome into 23 and 21 fragments, respectively, and the sums of the fragments gave a genome size of approximately 2.78 Mbp. Hybridization of probes for several cellulolytic and hemicellulolytic genes, as well as a 16S ribosomal RNA gene, gave complex signals on PFGE-separated restriction fragments. Mapping of the genome by use of linking clone hybridizations had been initiated. [ABSTRACT FROM AUTHOR]
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قاعدة البيانات: Complementary Index
الوصف
تدمد:03438651
DOI:10.1007/BF01576828