التفاصيل البيبلوغرافية
| العنوان: |
Calcium absorption by fish intestine: the involvement of ATP- and sodium-dependent calcium extrusion mechanisms. |
| المؤلفون: |
Flik, Gert, Schoenmakers, Theo, Groot, Jack, Os, Carel, Wendelaar Bonga, Sjoerd, Flik, G, Schoenmakers, T J, Groot, J A, van Os, C H, Wendelaar Bonga, S E |
| المصدر: |
Journal of Membrane Biology; 1990, Vol. 113 Issue 1, p13-22, 10p |
| مستخلص: |
Measurements of unidirectional calcium fluxes in stripped intestinal epithelium of the tilapia, Oreochromis mossambicus, in the presence of ouabain or in the absence of sodium indicated that calcium absorption via the fish intestine is sodium dependent. Active Ca2+ transport mechanisms in the enterocyte plasma membrane were analyzed. The maximum capacity of the ATP-dependent Ca2+ pump (Vm: 0.63 nmol.min-1.mg-1, Km:27 nM Ca2+) is calculated to be 2.17 nmol.min-1.mg-1, correcting for 29% inside-out oriented vesicles in the membrane preparation. The maximum capacity of the Na+/Ca2+ exchanger with high affinity for Ca2+ (Vm:7.2 nmol.min-1.mg-1, Km:181 nM Ca2+) is calculated to be 13.6 nmol.min-1.mg-1, correcting for 53% resealed vesicles and assuming symmetrical behavior of the Na+/Ca2+ exchanger. The high affinity for Ca2+ and the sixfold higher capacity of the exchanger compared to the ATPase suggest strongly that the Na+/Ca2+ exchanger will contribute substantially to Ca2+ extrusion in the fish enterocyte. Further evidence for an important contribution of Na+/Ca2+ exchange to Ca2+ extrusion was obtained from studies in which the simultaneous operation of ATP- and Na(+)-gradient-driven Ca2+ pumps in inside-out vesicles was evaluated. The fish enterocyte appears to present a model for a Ca2+ transporting cell, in which Na+/Ca2+ exchange activity with high affinity for Ca2+ extrudes Ca2+ from the cell. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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