التفاصيل البيبلوغرافية
| العنوان: |
A single nucleotide polymorphism assay for the identification of unisexual Ambystoma Salamanders. |
| المؤلفون: |
GREENWALD, KATHERINE R., LISLE GIBBS, H. |
| المصدر: |
Molecular Ecology Resources; Mar2012, Vol. 12 Issue 2, p354-362, 9p, 4 Charts, 2 Graphs |
| مصطلحات موضوعية: |
SALAMANDERS, AMBYSTOMA, PLOIDY, GYNOGENESIS, GENOMES |
| مستخلص: |
Unisexual (all female) salamanders in the genus Ambystoma are animals of variable ploidy (2N-5N) that reproduce via a unique system of 'leaky' gynogenesis. As a result, these salamanders have a diverse array of nuclear genome combinations from up to five sexual species: the blue-spotted ( A. laterale), Jefferson ( A. jeffersonianum), smallmouth ( A. texanum), tiger ( A. tigrinum) and streamside ( A. barbouri) salamanders. Identifying the genome complement, or biotype, is a critical first step in addressing a broad range of ecological and evolutionary questions about these salamanders. Previous work relied upon genome-related differences in allele size distributions for specific microsatellite loci, but overlap in these distributions among different genomes makes definitive identification and ploidy determination in unisexuals difficult or impossible. Here, we develop the first single nucleotide polymorphism assay for the identification of unisexual biotypes, based on species-specific nucleotide polymorphisms in noncoding DNA loci. Tests with simulated and natural unisexual DNA samples show that this method can accurately identify genome complement and estimate ploidy, making this a valuable tool for assessing the genome composition of unisexual samples. [ABSTRACT FROM AUTHOR] |
|
Copyright of Molecular Ecology Resources is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder