التفاصيل البيبلوغرافية
| العنوان: |
In vivo MRI of olfactory ensheathing cell grafts and regenerating axons in transplant mediated repair of the adult rat optic nerve. |
| المؤلفون: |
Sandvig, Ioanna, Thuen, Marte, Hoang, Linh, Olsen, Øystein, Sardella, Thomas CP, Brekken, Christian, Tvedt, Kåre E, Barnett, Susan C, Haraldseth, Olav, Berry, Martin, Sandvig, Axel |
| المصدر: |
NMR in Biomedicine; Apr2012, Vol. 25 Issue 4, p620-631, 12p |
| مستخلص: |
The purpose of the present study was to use magnetic resonance imaging (MRI) as a tool for monitoring transplant-mediated repair of the adult rat visual pathway. We labelled rat olfactory ensheathing cells (OECs) using micron-sized particles of iron oxide (MPIO) and transplanted them by: i) intravitreal injection ( ivit) and ii) intra-optic nerve (ON) injection ( iON) in adult rats with ON crush (ONC) injury. We applied T2-weighted MRI and manganese-enhanced MRI (MEMRI) to visualise transplanted cells and ON axons at specific times after injury and cell engraftment. Our findings demonstrate that ivit MPIO-labelled OECs are unequivocally detected by T2-weighted MRI in vivo and that the T1-weighted 3D FLASH sequence applied for MEMRI facilitates simultaneous visualisation of Mn2+−enhanced regenerating retinal ganglion cell (RGC) axons and MPIO-labelled OEC grafts. Furthermore, analysis of MRI data and ultrastructural findings supports the hypothesis that iON OEC transplants mediate regeneration and remyelination of RGC axons post injury. Copyright © 2011 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR] |
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