التفاصيل البيبلوغرافية
| العنوان: |
Rapid Detection of Norovirus from Fresh Lettuce Using Immunomagnetic Separation and a Quantum Dots Assay. |
| المؤلفون: |
HEE-MIN LEE, KWON, JOSEPH, JONG-SOON CHOI, KYEONG-HWAN LEE, SUNG YANG, SANG-MU KO, JAE-KEUN CHUNG, SE-YOUNG CHO, DUWOON KIM |
| المصدر: |
Journal of Food Protection; Apr2013, Vol. 76 Issue 4, p707-711, 5p |
| مصطلحات موضوعية: |
NOROVIRUSES, LETTUCE, FOOD contamination, IMMUNOMAGNETIC separation, QUANTUM dots, POLYMERASE chain reaction |
| مستخلص: |
Current molecular methods that include PCR have been used to detect norovirus in many food samples. However, the protocols require removing PCR inhibitors and incorporate time-consuming concentration steps to separate virus from analyte for rapid and sensitive detection of norovirus. We developed an immunomagnetic separation (IMS) and a quantum dots (QDs) assay to detect norovirus eluted. from fresh lettuce with Tris buffer containing 1 % beef extract (pH 9.5). IMS facilitated viral precipitation with a 10-min incubation, whereas virus concentration using polyethylene glycol (PEG) requires more than 3 h and an additional high-speed centrifugation step to precipitate virus before reverse transcription PCR (RT-PCR) analysis. The fluorescence intensity of QDs was detected qualitatively on norovirus dilutions of 10-1 to 10-3 in a stool suspension (100 RT-PCR units/ml). The results suggest that a fluorescence assay based on IMS and QDs is valid for detecting norovirus qualitatively according to fluorescent signal intensity within the same virus detection limit produced by IMS-RT-PCR and PEG-RT-PCR. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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