التفاصيل البيبلوغرافية
| العنوان: |
Maternal diabetes causes abnormal dynamic changes of endoplasmic reticulum during mouse oocyte maturation and early embryo development. |
| المؤلفون: |
Chun-Hui Zhang, Wei-Ping Qian, Shu-Tao Qi, Zhao-Jia Ge, Ling-Jiang Min, Xiu-Lang Zhu, Xin Huang, Jing-Ping Liu, Ying-Chun Ouyang, Yi Hou, Schatten, Heide, Qing-Yuan Sun |
| المصدر: |
Reproductive Biology & Endocrinology; 2013, Vol. 11 Issue 1, Special section p1-11, 11p |
| مصطلحات موضوعية: |
GESTATIONAL diabetes, ENDOPLASMIC reticulum, HUMAN embryo research, CONFOCAL microscopy, LABORATORY mice |
| مستخلص: |
Background: The adverse effects of maternal diabetes on oocyte maturation and embryo development have been reported. Methods: In this study, we used time-lapse live cell imaging confocal microscopy to investigate the dynamic changes of ER and the effects of diabetes on the ER's structural dynamics during oocyte maturation, fertilization and early embryo development. Results: We report that the ER first became remodeled into a dense ring around the developing MI spindle, and then surrounded the spindle during migration to the cortex. ER reorganization during mouse early embryo development was characterized by striking localization around the pronuclei in the equatorial section, in addition to larger areas of fluorescence deeper within the cytoplasm. In contrast, in diabetic mice, the ER displayed a significantly higher percentage of homogeneous distribution patterns throughout the entire ooplasm during oocyte maturation and early embryo development. In addition, a higher frequency of large ER aggregations was detected in GV oocytes and two cell embryos from diabetic mice. Conclusions: These results suggest that the diabetic condition adversely affects the ER distribution pattern during mouse oocyte maturation and early embryo development. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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