التفاصيل البيبلوغرافية
| العنوان: |
DNA repair is activated in early stages of p53-induced apoptosis. |
| المؤلفون: |
Geske, F J, Nelson, A C, Lieberman, R, Strange, R, Sun, T, Gerschenson, L E |
| المصدر: |
Cell Death & Differentiation; Apr2000, Vol. 7 Issue 4, p393, 9p |
| مصطلحات موضوعية: |
P53 antioncogene, APOPTOSIS, DNA repair |
| مستخلص: |
p53 is a complex molecule involved in apoptosis, cell cycle arrest, and DNA repair. Since apoptosis may play an important role in deletion of neoplastic cells, an understanding of the mechanism of p53-induced apoptosis may be critical for possible future therapeutic interventions. Recent evidence suggests that p53-induced apoptosis may involve members of the nucleotide excision repair (NER) family, linking these two cellular events. Our work using a temperature-sensitive p53 construct further analyzes p53-induced apoptosis in cultured murine mammary epithelial cells and also suggests that DNA repair plays a role in that process. Although p21 is induced in our system, apoptosis occurs without a detectable preceding G1 cell cycle arrest and independent of cellular alterations brought on by the temperature shift. In addition, clonogenic assays suggest that early stages of p53-induced apoptosis may be reversible upon removal of the apoptosis stimulus. As a possible explanation for this reversibility, our results show that general DNA repair activity increases early in p53-induced apoptosis. We also show that caspase-3 is activated at a timepoint when colony formation begins to drop, suggesting a possible mechanism for the point of no return in p53-induced apoptosis. [ABSTRACT FROM AUTHOR] |
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