التفاصيل البيبلوغرافية
| العنوان: |
An Investigation of Uterine Nitric Oxide Production in Mares Susceptible and Resistant to Persistent Breeding-Induced Endometritis and the Effects of Immunomodulation. |
| المؤلفون: |
Woodward, EM, Christoffersen, M, Campos, J, Horohov, DW, Scoggin, KE, Squires, E, Troedsson, MHT |
| المصدر: |
Reproduction in Domestic Animals; Aug2013, Vol. 48 Issue 4, p554-561, 8p |
| مصطلحات موضوعية: |
UTERUS physiology, ENDOMETRITIS, NITRIC-oxide synthases, IMMUNOREGULATION, MARES, DISEASE susceptibility, DISEASES, REPRODUCTION |
| مستخلص: |
Contents The first objective of this study was to evaluate intrauterine nitric oxide ( NO) and endometrial inducible NO synthase (i NOS) in mares susceptible or resistant to persistent breeding-induced endometritis ( PBIE) within 24 h after breeding. Mares susceptible (n = 6) or resistant (n = 6) to PBIE were inseminated over five cycles, and uterine secretions and endometrial biopsies were collected before and 2, 6, 12 and 24 h after insemination. Uterine secretions were analysed for NO and biopsies were analyzed for i NOS expression. A second experiment evaluated the effect of treatment with dexamethasone or mycobacterial cell wall extract ( MCWE) on uterine NO production and endometrial i NOS m RNA expression. Six susceptible mares were inseminated over three cycles with (i) killed spermatozoa without treatment (control), (ii) killed spermatozoa with 50 mg of dexamethasone IV or (iii) MCWE IV 24 h prior to insemination with killed spermatozoa. Six resistant mares were inseminated with killed spermatozoa as a control. Six hours after breeding, uterine biopsies and secretions were collected and evaluated for NO and i NOS m RNA. In Experiment 1, resistant mares had an increase in i NOS m RNA expression 2 h post-breeding compared to baseline (p = 0.045), 12 h (p = 0.014) and 24 h (p = 0.001). Susceptible mares had higher expression 2 h compared to 6 h (p = 0.046). No differences were observed in m RNA or protein expression of i NOS between resistant and susceptible mares. Resistant mares had a relatively steady amount of total intrauterine NO over 24 h, while susceptible mares had an increase over time, with a significantly higher increase in total NO than resistant mares at 6 (p = 0.04) and 12 h (p = 0.032). In Experiment 2, no differences were observed for i NOS m RNA expression. Susceptible mares had increased NO when compared to resistant mares (p = 0.008) and MCWE decreased NO (p = 0.047). [ABSTRACT FROM AUTHOR] |
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