التفاصيل البيبلوغرافية
| العنوان: |
Mucosal Mast Cells Response in the Jejunum of Ascaridia galli-Infected Laying Hens. |
| المؤلفون: |
Darmawi, Balqisa, U., Hambal, M., Tiuria, R., Frengki, Priosoeryanto, B. P. |
| المصدر: |
Media Peternakan; Aug2013, Vol. 36 Issue 2, p113-119, 7p |
| مصطلحات موضوعية: |
MAST cells, INTESTINAL diseases, PARASITIC nematodes in mammals, PARASITIC diseases, ASCARIASIS, HENS, JEJUNUM |
| Abstract (English): |
Intestinal defense mechanism against helminthes parasitic nematode to be associated with mucosal mast cells reaction. The aim of this research was to examine the effect of infection by Ascaridia galli parasite to trigger mucosal defense based on mucosal mast cells response in laying hens. Amount of ten head laying hens 12-wk old were divided into two groups containing five chickens of each. The first group, chickens were left as un-infected controls. The second group, chickens were infected orally with 1,000 embryonated eggs of A. galli. Mucosal mast cell responses were assayed by in situ jejunal mast cell counts in stained serial histological sections with Alcian blue (pH 0.3) and Safranin-O (pH 0.1) of the jejunum. Mucosal mast cells response were observed and counted on days 14 post infection. The result showed that A. galli infection was able to increase significantly (P<0.05) mast cells response. This research concluded that the A. galli infection can trigger the involment of mucosal mast cells response in jejunal defense of laying hens against parasitic diseases caused by A. galli. [ABSTRACT FROM AUTHOR] |
| Abstract (Indonesian): |
Mekanisme pertahanan intestinal terhadap cacing nematoda parasitik berkaitan dengan reaksi sel mast mukosa. Tujuan riset ini adalah menguji pengaruh infeksi parasit Ascaridia galli dalam merangsang pertahanan mukosa berdasarkan respons sel mast mukosa pada ayam petelur. Sebanyak sepuluh ekor ayam petelur yang berumur 12 minggu dibagi menjadi dua kelompok, masing-masing kelompok terdiri atas lima ekor ayam. Kelompok pertama, ayam tidak diinfeksi sebagai kontrol. Kelompok kedua, ayam diinfeksi melalui oral dengan 1.000 telur berembrio A. galli. Respons sel mast mukosa diuji in situ dengan menghitung sel mast pada jejunum melalui pewarnaan serial histologik dengan Alcian blue( pH 0,3) dan Safranin-O pH 0,1) dari sediaan preparat jejunum. Respons sel mast mukosa diamati dan dihitung pada hari ke-14 pascainfeksi. Hasil menunjukkan bahwa infeksi A. galli dapat meningkatkan respons sel mast mukosa secara signifikan (P<0,05). Riset ini menyimpulkan bahwa infeksi cacing A. galli dapat merangsang keterlibatan respons sel mast mukosa pada pertahanan jejunum ayam petelur terhadap penyakit parasitik yang disebabkan oleh A. galli. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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