التفاصيل البيبلوغرافية
| العنوان: |
Expression of aberrant HLA-B27 molecules is dependent on B27 dosage and peptide supply. |
| المؤلفون: |
McHugh, Kirsty, Rysnik, Oliwia, Kollnberger, Simon, Shaw, Jacqueline, Utriainen, Lotta, Mohammad Hussein Al-Mossawi, Payeli, Sravan, Marroquin, Osiris, Milling, Simon, Renner, Christoph, Bowness, Paul |
| المصدر: |
Annals of the Rheumatic Diseases; Apr2014, Vol. 73 Issue 4, p763-770, 8p, 1 Diagram, 1 Chart, 4 Graphs |
| مستخلص: |
Objectives Cellular expression of non-classical forms of human leukocyte antigen (HLA)-B27 (NC-B27) may be involved in spondyloarthritis (SpA) pathogenesis. We used a novel B27-specific monoclonal antibody, HD6, to ask if B27 transgenic (TG) rat splenocytes express these NC-B27 molecules. We also investigated whether B27-binding peptides could affect the expression and functional immune recognition of HD6-reactive B27 molecules. Methods Splenocytes from B27-TG, B7-TG and non-transgenic rats, and HLA-B27+ cell lines were stained with monoclonal antibodies recognising classical (ME-1, HLA-ABC-m1) and non-classical (HD6, HC10) B27. Cells were further cultured in the presence of HLA-B27-binding peptides, or subjected to brief low pH treatment prior to mAb staining and/or immunoprecipitation or co-culture with KIR3DL2-CD3ε-expressing Jurkat reporter cells. Results HD6-reactive molecules were detected in the majority of adult B27-TG rat splenocyte cell subsets, increasing with age and concomitant increased B27 expression. HD6 staining was inhibited by incubation with B27-binding peptides and induced by low pH treatment. HD6 staining correlated with KIR3DL2-CD3ε-expressing Jurkat reporter cell activity. Thus, IL-2 production was decreased when B27-expressing antigen-presenting cells were preincubated with B27-binding peptides, but increased following pretreatment with low pH buffer. Conclusions Surface expression of HD6-reactive B27 molecules on B27-TG rat splenocytes is consistent with a pathogenic role for NC-B27 in SpA. Interaction of NC-B27 with innate immune receptors could be critical in SpA pathogenesis, and we show that this may be influenced by the availability and composition of the B27-binding peptide pool. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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