التفاصيل البيبلوغرافية
| العنوان: |
Effects of THAP11 on Erythroid Differentiation and Megakaryocytic Differentiation of K562 Cells. |
| المؤلفون: |
Kong, Xiang-Zhen, Yin, Rong-Hua, Ning, Hong-Mei, Zheng, Wei-Wei, Dong, Xiao-Ming, Yang, Yang, Xu, Fei-Fei, Li, Jian-Jie, Zhan, Yi-Qun, Yu, Miao, Ge, Chang-Hui, Zhang, Jian-Hong, Chen, Hui, Li, Chang-Yan, Yang, Xiao-Ming |
| المصدر: |
PLoS ONE; Mar2014, Vol. 9 Issue 3, p1-9, 9p |
| مصطلحات موضوعية: |
CELL differentiation, MEGAKARYOCYTE differentiation, TRANSCRIPTION factors, CELL growth, ERYTHROCYTES, HEMATOPOIETIC stem cells |
| مستخلص: |
Hematopoiesis is a complex process regulated by sets of transcription factors in a stage-specific and context-dependent manner. THAP11 is a transcription factor involved in cell growth, ES cell pluripotency, and embryogenesis. Here we showed that THAP11 was down-regulated during erythroid differentiation but up-regulated during megakaryocytic differentiation of cord blood CD34+ cells. Overexpression of THAP11 in K562 cells inhibited the erythroid differentiation induced by hemin with decreased numbers of benzidine-positive cells and decreased mRNA levels of α-globin (HBA) and glycophorin A (GPA), and knockdown of THAP11 enhanced the erythroid differentiation. Conversely, THAP11 overexpression accelerated the megakaryocytic differentiation induced by phorbol myristate acetate (PMA) with increased percentage of CD41+ cells, increased numbers of 4N cells, and elevated CD61 mRNA levels, and THAP11 knockdown attenuated the megakaryocytic differentiation. The expression levels of transcription factors such as c-Myc, c-Myb, GATA-2, and Fli1 were changed by THAP11 overexpression. In this way, our results suggested that THAP11 reversibly regulated erythroid and megakaryocytic differentiation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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