التفاصيل البيبلوغرافية
| العنوان: |
Rare allele of HvLox- 1 associated with lipoxygenase activity in barley ( Hordeum vulgare L.). |
| المؤلفون: |
Guo, Ganggang, Dondup, Dawa, Yuan, Xingmiao, Gu, Fanghong, Wang, Deliang, Jia, Fengchao, Lin, Zhiping, Baum, Michael, Zhang, Jing |
| المصدر: |
Theoretical & Applied Genetics; Oct2014, Vol. 127 Issue 10, p2095-2103, 9p |
| مصطلحات موضوعية: |
ALLELES in plants, LIPOXYGENASES, BARLEY enzymes, GENETIC markers in plants, SINGLE nucleotide polymorphisms, PLANT breeding, PLANT germplasm |
| مستخلص: |
Key message: Identification and allele-specific marker development of a functional SNP of HvLox - 1 which associated with barley lipoxygenase activity. Abstract: Improving the stability of the flavor of beer is one of the main objectives in breeding barley for malting, and lipoxygenase-1 (LOX-1) is a key enzyme controlling this trait. In this study, a modified LOX activity assay was used for null LOX-1 mutant screening. Four barley landraces with no detected level of LOX-1 activity were screened from 1,083 barley germplasm accessions from China. The genomic sequence diversity of the HvLox- 1 gene of the four null LOX-1 Chinese landraces was compared with that of a further 76 accessions. A total of 104 nucleotide polymorphisms were found, which contained 83 single-nucleotide polymorphisms (SNPs), 7 multiple-nucleotide polymorphisms, and 14 insertions and deletions. Most notably, we found a rare C/G mutation (SNP-61) in the second intron which led to null LOX-1 activity through an altered splicing acceptor site. In addition, an allele-specific polymerase chain reaction marker was developed for the genotyping of SNP-61, which could be used in breeding programs for barley to be used for malting. The objective was to improve beer quality. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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