التفاصيل البيبلوغرافية
| العنوان: |
Transgene expression in the rhesus cervix mediated by an adenovirus expressing beta-galactosidase. |
| المؤلفون: |
Mitchell, M F, Hamada, K, Sastry, K J, Sarkar, A, Tortolero-Luna, G, Wharton, J T, Roth, J A |
| المصدر: |
American Journal of Obstetrics & Gynecology; Apr96, Vol. 174 Issue 4, p1094-1101, 8p |
| مصطلحات موضوعية: |
ANIMAL experimentation, CERVIX uteri, COMPARATIVE studies, GENE expression, GENES, GENETIC techniques, GLYCOSIDASES, IMMUNOGLOBULINS, RESEARCH methodology, MEDICAL cooperation, PRIMATES, RESEARCH, VIRAL antibodies, VIRUSES, EVALUATION research |
| مستخلص: |
Objectives: More than 90% of cervical cancers are positive for human papillomavirus, which functionally represses p53 and pRb. The remainder have been found to contain p53 mutations. Gene therapy involves insertion of a functioning gene into a patient to correct a genetic abnormality.Study Design: The ability of a beta-galactosidase adenovirus to mediate transgene expression in the rhesus cervix was evaluated. Three different doses and two different entry techniques of virus were investigated.Results: The ideal dose determined by X-galactosidase staining was 2 x 10(10) plaque-forming units, and the injection method yielded better staining than did abrasion with topical application. Increased adenoviral-specific immunoglobulin G antibody response in the injected monkeys confirmed the results.Conclusion: High transduction efficiency by use of adenoviral vectors can be achieved in the cervix. Reversing the effects of human papillomavirus and p53 mutations with gene therapy may become a novel therapy for invasive and preinvasive cervical cancer. [ABSTRACT FROM AUTHOR] |
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