التفاصيل البيبلوغرافية
| العنوان: |
Hypoxia-Induced Upregulation of Matrix Metalloproteinase 9 Increases Basement Membrane Degradation by Downregulating Collagen Type IV Alpha 1 Chain. |
| المؤلفون: |
Ming-Ming ZHU, Yi MA, Meng TANG, Li PAN, Wen-Ling LIU |
| المصدر: |
Physiological Research; Dec2022, Vol. 71 Issue 6, p825-834, 10p |
| مصطلحات موضوعية: |
HYPOXEMIA, COLLAGEN, MATRIX metalloproteinases, BASAL lamina, IMMUNOHISTOCHEMISTRY |
| مستخلص: |
Hypoxia can cause basement membrane (BM) degradation in tissues. Matrix metalloproteinase 9 (MMP-9) is involved in various human cancers as well as BM degradation by downregulating type IV collagen (COL4). This study investigated the role of MMP-9 in hypoxia-mediated BM degradation in rat bone marrow based on its regulation of collagen type IV alpha 1 chain (COL4A1). Eighty male rats were randomly divided into four groups based on exposure to hypoxic conditions at a simulated altitude of 7,000 m, control (normoxia) and 3, 7, and 10 days of hypoxia exposure. BM degradation in bone marrow was determined by transmission electron microscopy. MMP-9 levels were assessed by western blot and real-time PCR, and COL4A1 levels were assessed by western blot and immunohistochemistry. Microvessels BMs in bone marrow exposed to acute hypoxia were observed by electron microscopy. MMP-9 expression increased, COL4A1 protein expression decreased, and BM degradation occurred in the 10-, 7-, and 3-day hypoxia groups compared with that in the control group (all P<0.05). Hypoxia increased MMP-9 levels, which in turn downregulated COL4A1, thereby increasing BM degradation. MMP-9 upregulation significantly promoted BM degradation and COL4A1 downregulation. Our results suggest that MMP-9 is related to acute hypoxia-induced BM degradation in bone marrow by regulating COL4A1. [ABSTRACT FROM AUTHOR] |
|
Copyright of Physiological Research is the property of Institute of Physiology, Academy of Sciences of the Czech Republic and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Supplemental Index |
Full Text Finder