التفاصيل البيبلوغرافية
| العنوان: |
Outer membrane protein BamA-based ELISA differentiates Salmonella-vaccinated chickens from naturally infected chickens. |
| المؤلفون: |
ARIBAM, Swarmistha Devi, Momoko NAKAYAMA, Yohsuke OGAWA, Yoshihiro SHIMOJI, Masahiro EGUCHI |
| المصدر: |
Journal of Veterinary Medical Science; 2023, Vol. 85 Issue 8, p809-812, 4p |
| مصطلحات موضوعية: |
MEMBRANE proteins, LEGHORN chicken, SALMONELLA diseases, CHICKENS, ANTIBODY titer |
| مصطلحات جغرافية: |
ALABAMA |
| مستخلص: |
Salmonella often causes subclinical infection in chickens, but antibody tests can find infected individuals and control the spread of infection. In this study, the S. Typhimurium-specific outer membrane, ß-barrel assembly machinery protein A (BamA), was overexpressed in Escherichia coli and purified as a coating antigen to develop a BamA-based enzyme-linked immuno sorbent assay for detecting Salmonella infection. The presence of anti-BamA IgG was detected in the sera of infected BALB/c mice, but not in that of heat-killed Sa/mone//a-vaccinated mice. The assay was validated using White Leghorn chickens and showed similar results. The detection of BamA antibodies in the sera can differentiate infected chickens from vaccinated chickens. This assay will be useful for monitoring Salmonella infection in chickens and possibly in other animals. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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