التفاصيل البيبلوغرافية
| العنوان: |
Promoter generation for the chimeric sex-determining gene dm-W in Xenopus frogs. |
| المؤلفون: |
Shun Hayashi, Kei Tamura, Daisuke Tsukamoto, Yusaku Ogita, Nobuhiko Takamatsu, Michihiko Ito |
| المصدر: |
Genes & Genetic Systems; Apr2023, Vol. 98 Issue 2, p53-60, 8p |
| مصطلحات موضوعية: |
XENOPUS, GENETIC sex determination, XENOPUS laevis, FROGS, SEX determination, CHROMOSOME duplication |
| مستخلص: |
Many sex-determining genes (SDGs) were generated as neofunctionalized genes through duplication and/or mutation of gonadal formation-related genes. We previously identified dm-W as an SDG in the African clawed frog Xenopus laevis and found that a partial duplication of the masculinization gene dmrt1 created the neofunctionalized dm-W after allotetraploidization by interspecific hybridization. The allotetraploid Xenopus species have two dmrt1 genes, dmrt1.L and dmrt1.S. Xenopus laevis dm-W has four exons: two dmrt1.S-derived exons (exons 2 and 3) and two other exons (noncoding exon 1 and exon 4). Our recent work revealed that exon 4 originated from a DNA transposon, hAT-10. Here, to clarify when and how the noncoding exon 1 and its coexisting promoter evolved during the establishment of dm-W after allotetraploidization, we newly determined nucleotide sequences of the dm-W promoter region from two other allotetraploid species, X. largeni and X. petersii, and performed an evolutionary analysis. We found that dm-W acquired a new exon 1 and TATA-type promoter in the common ancestor of the three allotetraploid Xenopus species, resulting in the deletion of the dmrt1.S-derived TATA-less promoter. In addition, we demonstrated that the TATA box contributes to dm-W promoter activity in cultured cells. Collectively, these findings suggest that this novel TATA-type promoter was important for the establishment of dm-W as a sex-determining gene, followed by the degeneration of the preexisting promoter. [ABSTRACT FROM AUTHOR] |
|
Copyright of Genes & Genetic Systems is the property of Genetics Society of Japan and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Supplemental Index |
Full Text Finder