التفاصيل البيبلوغرافية
| العنوان: |
Synergistic enhancement of glucagon-like peptide-1 release by ?-aminobutyric acid and L-phenylalanine in enteroendocrine cells--searching active ingredients in a water extract of corn zein protein. |
| المؤلفون: |
Hiroki Noguchi, Noriyuki Kohda, Hiroshi Hara, Tohru Hira |
| المصدر: |
Bioscience, Biotechnology & Biochemistry; Dec2023, Vol. 87 Issue 12, p1505-1513, 9p |
| مصطلحات موضوعية: |
ENTEROENDOCRINE cells, PHENYLALANINE, LIQUID chromatography-mass spectrometry, GABA antagonists, HIGH performance liquid chromatography, CORN |
| مستخلص: |
This study investigated the glucagon-like peptide-1 (GLP-1)-releasing activity of an aqueous extract (ZeinS) from corn zein protein and aimed to identify the active compounds responsible for this activity. Glucagon-like peptide-1-releasing activity was evaluated using a murine enteroendocrine cell line (GLUTag). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed on purified fractions of ZeinS to identify active molecules. ZeinS stimulated more GLP-1 secretion from GLUTag cells compared to zein hydrolysate. Fractions displaying biological activity were determined by solid-phase extraction and high-performance liquid chromatography (HPLC) fractionation. Subsequent LC-MS/MS analysis identified several amino acids in the active fractions of ZeinS. In particular, γ-aminobutyric acid (GABA) exhibited significant GLP-1-releasing activity both alone and synergistically with L-phenylalanine (Phe). Moreover, ZeinS-induced GLP-1 secretion was attenuated by antagonists for the GABA receptor and calcium sensing receptor. These results demonstrate that GABA and Phe identified in ZeinS synergistically stimulate GLP-1 secretion in enteroendocrine cells. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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