التفاصيل البيبلوغرافية
| العنوان: |
Preparation and characterization of spiked gold nanobipyramids and its antibacterial effect on methicillin-resistant Staphylococcus aureus and methicillin-sensitive Staphylococcus aureus. |
| المؤلفون: |
Phat Trong Huynh, Khanh Thi Le Tran, Tham Thi Hong Nguyen, Vinh Quang Lam, Ngan Thi Kim Phan, Thanh Vo Ke Ngo |
| المصدر: |
Journal of Genetic Engineering & Biotechnology; 11/15/2023, Vol. 21 Issue 1, p1-11, 11p |
| مصطلحات موضوعية: |
METHICILLIN-resistant staphylococcus aureus, STAPHYLOCOCCUS aureus, DRUG resistance in bacteria, GOLD, SCANNING electron microscopy, GOLD nanoparticles, CHEMICAL purification |
| مستخلص: |
Background This paper reports the preparation of a new family of spiked gold nanoparticles, spiked gold nanobipyramids (SNBPs). This protocol includes the process to synthesize gold nanobipyramids (NBPs) using combined seed-mediated and microwave-assisted method and procedure to form spikes on whole surface of gold nanobipyramid. We also evaluated the antibacterial activity against both methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) in various concentrations of SNBPs and NBPs by well diffusion assay, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) determination. The effect of SNBPs on exposed bacteria was observed by scanning electron microscopy. Results The UV-Vis of purified NBPs exhibited two absorption bands located at 550 nm and 849 nm with yield of bipyramidal particles more than 90%. The average size of NBPs was 76.33 ± 10.11 nm in length and 26.57 ± 2.25 nm in diameter, respectively, while SNBPs were prolongated in length and achieved 182.37 ± 21.74 nm with multibranches protruding whole surface areas. In antibacterial evaluations, SNBPs and NBPs showed antibacterial activity with MIC of 6.25 μl/ml and 12.5 μl/ml, respectively, for MSSA while 12.5 μl/ml and 25 μl/ml, respectively, for MRSA. Besides, MBC values of SNBPs and NBPs were found to be 12.5 μl/ml and 25 μl/ml, respectively, against MSSA while 25 μl/ml and 50 μl/ml, respectively, against MRSA. Furthermore, scanning electron microscopy observation showed the mechanism that SNBPs damaged the outer membrane, released cytoplasm, and altered the normal morphology of MRSA and MSSA, leading to bacterial death. Conclusions This report suggests that these SNBPs are potential antibacterial agents that can be applied as antibacterial materials to inhibit the growth of human bacterial pathogen infections related to antibiotic-resistant bacteria. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Genetic Engineering & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Supplemental Index |
Find this article in full text from Springer Verlag. 
Full Text Finder