التفاصيل البيبلوغرافية
| العنوان: |
RNAscope: a novel method for the detection of Heterobilharzia americana ova in canine liver. |
| المؤلفون: |
Devora, Priscilla A., Johnston, Andrea N. |
| المصدر: |
Journal of Veterinary Diagnostic Investigation; Jul2024, Vol. 36 Issue 4, p538-542, 5p |
| مصطلحات موضوعية: |
OVUM, NUCLEIC acid hybridization, LIVER flukes, IN situ hybridization, SCHISTOSOMIASIS, EGGS |
| مستخلص: |
Canine schistosomiasis caused by Heterobilharzia americana can lead to severe morbidity and eventual mortality, in part due to the deposition of fluke ova in the liver and gastrointestinal tract, which promotes an influx of peri-ova inflammatory cells. Although fluke eggs can be identified in H&E-stained histologic sections, cases exist in which only fragments of the ova persist, or the egg is obscured by inflammatory infiltrates, which can confound definitive histologic diagnosis. Unfortunately, antibodies specific to Heterobilharzia are not commercially available for immunohistochemical labeling. Therefore, we aimed to use an RNA in situ hybridization strategy to fluorescently label Heterobilharzia ova. Using the H. americana 18S rRNA sequence, we developed an RNA probe and validated its performance on archival formalin-fixed, paraffin-embedded canine tissue. A positive signal was observed for all identifiable ova, fragmented and whole. Use of this methodology could aid understanding of the pathogenesis of H. americana infection in dogs. This technique augments standard diagnostic methodology, enabling spatial colocalization of fluke ova and inflammatory infiltrates when using fluorescent techniques. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Veterinary Diagnostic Investigation is the property of Sage Publications Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Supplemental Index |
Full Text Finder