التفاصيل البيبلوغرافية
| العنوان: |
Identification of Lymphoma-Associated Antigens Using SEREX. |
| المؤلفون: |
Illidge, Tim, Johnson, Peter W. M., Liggins, Amanda P., Guinn, Barbara A., Banham, Alison H. |
| المصدر: |
Lymphoma: Methods in Molecular Medicine; 2005, p109-128, 20p |
| مستخلص: |
Serological analysis of recombinant complementary deoxyribonucleic acid (cDNA) expression libraries (SEREX) is a powerful approach to identify immunogenic cancer-associated proteins using antibodies that are naturally present in the serum of cancer patients. This technique involves the screening of a relevant cDNA expression library with patient serum that has been cleaned to remove any antibodies that may recognize bacterial and/or viral proteins. Once antigens have been identified and their reactivity has been confirmed with a second round of screening, the gene encoding the protein can be sequenced and identified. Antigens can then be screened with a panel of allogenic sera from other patients and control individuals. This identifies disease-specific antigens, which may be useful diagnostic markers or, alternatively, targets for immunotherapy. This chapter describes the SEREX methodology in full. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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