دورية
Viable Porcine Hepatocytes from Slaughterhouse Organs
| العنوان: | Viable Porcine Hepatocytes from Slaughterhouse Organs |
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| المؤلفون: | Hg, Koebe, Mueller-Hoecker, J., Koebe, S., Fw, Schildberg |
| المصدر: | Artificial Cells, Blood Substitutes, and Biotechnology; 1995, Vol. 23 Issue: 5 p613-626, 14p |
| مستخلص: | A modified enzymatic isolation technique for the successful harvesting of porcine liver cells from slaughterhouse organs is introduced. Digestion of the left medial liver lobe (n=74) resulted in 1.0 ±0.3 ± 10E7 viable hepatocytes per gram tissue and an overall yield of 1.92 ±0.5 ± 10E9 cells per isolation (viability: 93 ±2%).Morphological integrity of hepatocytes in long term immobilization culture systems was assessed by light and electron microscopic follow up. Stable DNA-contents and low alanineamino-transferase (ALAT) release were measured after early culture adaptation. Urea production under NH4Cl, Albumin secretion, total bile acid synthesis and a follow up of 7-ethoxicoumarin o-deethylase (ECOD) activity demonstrated functional activity and maintenance of Type IA1 cytochrome P450 (CYP450) dependent metabolism in cultured hepatocytes for at least 10 days.In contrast to published results, where hepatocytes have been mainly isolated from anaesthetized animals we could not see any disadvantages in the use of liver cells from slaughterhouse organs.Hepatocytes from slaughtered pigs represent an unlimited, reliable and inexpensive resource of viable cell material for all fields of applied research, including investigations on hepatic metabolism or the development of hybrid artificial liver support devices. The technique could also help to reduce animal experiments for mere cell harvesting purposes. We conclude that -wherever possible- porcine hepatocytes should be obtained from slaughterhouse organs instead of ex vivo cell isolation. |
| قاعدة البيانات: | Supplemental Index |
PDF full text from Publisher | تدمد: | 10731199 15324184 |
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| DOI: | 10.3109/10731199509117976 |