دورية
Maintenance of liver function in long term culture of hepatocytes following In Vitro or In Vivo Ha-rasEJ transfection
| العنوان: | Maintenance of liver function in long term culture of hepatocytes following In Vitro or In Vivo Ha-rasEJ transfection |
|---|---|
| المؤلفون: | Fischbach, Michel, Cao, Hanwei, Ibanez, Miguel Diez, Tsaconas, Christos, Alouani, Sami, Montandon, Frédéric, Baraka, Mohammed El, Padieu, Prudent, Dreano, Michel, Chessebeuf-Padieu, Martine |
| المصدر: | Cell Biology and Toxicology; October 1991, Vol. 7 Issue: 4 p327-345, 19p |
| مستخلص: | Collagenase isolated rat hepatocytes were transfected with liposome encapsulated pEJ (LE-pEJ), a plasmid carrying the human cellular activated Ha-rasEJ oncogene. A proliferative cell line was cloned from these cells transfected in vitro. It secreted per day 0.87 µg albumin and 0.32 µg transferrin per 106 cells, and 11.06 nmol free and conjugated bile acids (BA) per mg protein. Also, it metabolized 2-acetylaminoflourene (2-AFAF) into N- and ring-hydroxylated metabolites and 2-aminofluorene at rates of 1.50, 9.73, and 1.98 nmol/mg cell protein/24 hr, respectively. Rats were i.v. injected with both LE-pEJ and LE-p17hGHnneo carrying the hGH cDNA gene, and secreted hGH in the plasma which induced the synthesis of anti-hGH antibodies. A cell line was cloned from cultures of primary hepatocytes isolated from the liver of transfected rats. After 2 to 3 months in culture, this cell line secreted per day 18.9 µg albumin and 11.0 µg transferrin per 106 cells, 38.75 nmol total BA per mg cell protein, and up to 31 ng hGHper 106 cells without cloning hGH recombinant cells. A 24 hr control culture of primary hepatocytes isolated from non transfected rats secreted 25.5 µg albumin and 11.7 µg transferrin per 106 cells, and produced 21.64 nmol total BA and 2.13 nmol N-OH-2-AFAF per mg cell protien. Hence, Ha-rasEJ transfection of either hepatocytes in vitro or liver cells in vivo, initiated cell cycles leading to presumptive proliferating hepatocytes which express liver function. |
| قاعدة البيانات: | Supplemental Index |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 07422091 15736822 |
|---|---|
| DOI: | 10.1007/BF00124069 |