دورية
Studies on ouabain-like endogenous natriuretic factors in human urine. Inhibition of Na-K-ATPase and3H-Ouabain binding
| العنوان: | Studies on ouabain-like endogenous natriuretic factors in human urine. Inhibition of Na-K-ATPase and3H-Ouabain binding |
|---|---|
| المؤلفون: | Kramer, H. J., Baecker, A., Weiler, E., Liddiard, C. |
| المصدر: | Journal of Molecular Medicine; August 1986, Vol. 64 Issue: 16 p760-766, 7p |
| مستخلص: | Summary An endogenous inhibitor of sodium transport and of the Na-K-ATPase enzyme was previously detected in the small molecular weight postsalt fraction SIV of serum from saline-loaded rats after gel filtration on Sephadex G-25. In addition, a natriuretic factor present in this fraction of urine from salt-loaded subjects was found to bind to a specific digoxin antibody. Therefore, in the present study the small molecular weight natriuretic and digoxin antibody-binding activities present in the urine of salt-loaded healthy volunteers were purified by reverse-phase chromatography and by immunoprecipitation with the digoxin antibody and were studied for their in vitro effects on Na-K-ATPase derived from hog cerebral cortex. Na-K-ATPase inhibitory activities were found to roughly parallel the natriuretic activities at the various stages of purification. After reverse-phase chromatography, the material of fraction SIV which was bound to the digoxin antibody revealed the highest specific natriuretic activity of 3.95 ± 0.29 µmol/min × mg injected material and showed strongest inhibition of the enzyme with I |
| قاعدة البيانات: | Supplemental Index |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 09462716 14321440 |
|---|---|
| DOI: | 10.1007/BF01734344 |