دورية
A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon.
| العنوان: | A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon. |
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| المؤلفون: | Heisenberg, C P, Houart, C, Take-Uchi, M, Rauch, G J, Young, N, Coutinho, P, Masai, I, Caneparo, L, Concha, M L, Geisler, R, Dale, T C, Wilson, S W, Stemple, D L |
| المصدر: | Genes & Development; June 2001, Vol. 15 Issue: 11 p1427-1434, 8p |
| مستخلص: | Zebrafish embryos homozygous for the masterblind (mbl) mutation exhibit a striking phenotype in which the eyes and telencephalon are reduced or absent and diencephalic fates expand to the front of the brain. Here we show that mbl(-/-) embryos carry an amino-acid change at a conserved site in the Wnt pathway scaffolding protein, Axin1. The amino-acid substitution present in the mbl allele abolishes the binding of Axin to Gsk3 and affects Tcf-dependent transcription. Therefore, Gsk3 activity may be decreased in mbl(-/-) embryos and in support of this possibility, overexpression of either wild-type Axin1 or Gsk3beta can restore eye and telencephalic fates to mbl(-/-) embryos. Our data reveal a crucial role for Axin1-dependent inhibition of the Wnt pathway in the early regional subdivision of the anterior neural plate into telencephalic, diencephalic, and eye-forming territories. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 08909369 15495477 |
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| DOI: | 10.1101/gad.194301 |