التفاصيل البيبلوغرافية
| العنوان: |
Functional characterisation of the Drosophila5-HT dro1 and 5-HT dro2Bserotonin receptors in insect cells: activation of a G α s-like protein by 5-HT dro1 but lack of coupling to inhibitory G-proteins by 5-HT dro2B |
| المؤلفون: |
Obosi, Louis A., Schuette, Diana G., Europe-Finner, G.Nicholas, Beadle, David J., Hen, René, King, Linda A., Bermudez, Isabel |
| المصدر: |
FEBS Letters; January 1996, Vol. 381 Issue: 3 p233-236, 4p |
| مستخلص: |
Insect cells are routinely used for the production of receptor proteins. Expression of the Drosophila5-HT dro1 serotonin receptor resulted in positive coupling of the receptor to adenylyl cyclase via the G α sG-protein subtype. The Drosophila5-HT dro2Breceptor stimulated the metabolism of inositol phospholipid via a pertussis toxin-insensitive G-protein, but exhibited no detectable inhibition of adenylyl cyclase. Immunoblot analysis of the endogenous G-proteins revealed that Sf9 cells lack the G-protein subtypes G α i1–3 and G α0, but express the subtypes G α sand G α q. |
| قاعدة البيانات: |
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