دورية
A spontaneous Escherichia coliK12 mutant which inhibits the excision‐reintegration process of Mu gem2ts
| العنوان: | A spontaneous Escherichia coliK12 mutant which inhibits the excision‐reintegration process of Mu gem2ts |
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| المؤلفون: | Lallo, Gustavo, Fabozzi, Giulia, Ghelardini, Patrizia, Paolozzi, Luciano |
| المصدر: | FEMS Microbiology Letters; September 1997, Vol. 154 Issue: 1 p59-64, 6p |
| مستخلص: | Escherichia coliK12 strains lysogenic for Mu gem2ts with the prophage inserted in a target gene (i.e., lacZ::Mu gem2ts lysogenic strains) revert to Lac+by prophage precise excision with a relatively high frequency (about 1×10−6). The revertants obtained are still lysogens with the prophage inserted elsewhere in the bacterial chromosome. We have observed that, with the time of storage in stabs, bacterial cultures lysogenic for Mu gem2ts lose the ability to excise the prophage. The mutation responsible for this effect was co‐transducible with the gyrBgene. After the removal of the prophage by P1 virtransduction from these strains, one randomly chosen clone, R3538, was further analyzed. It shows an increment of DNA supercoiling of plasmid pAT153, used as a reporter, and a reduced β‐galactosidase activity. On the other hand, R3538 is totally permissive to both lytic and lysogenic cycles of bacteriophage Mu. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 03781097 15746968 |
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| DOI: | 10.1111/j.1574-6968.1997.tb12624.x |