In situ hybridization in paracoccidioidomycosis

التفاصيل البيبلوغرافية
العنوان: In situ hybridization in paracoccidioidomycosis
المؤلفون: De Brito, T., Sandhu, G. S., Kline, B. C., Aleff, R. A., Sandoval, M. P., Santos, R. T., Brandão, A. A. H., Lacaz, C. S.
المصدر: Medical Mycology; January 1999, Vol. 37 Issue: 3 p207-207, 1p
مستخلص: In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.
قاعدة البيانات: Supplemental Index
الوصف
تدمد:13693786
14602709
DOI:10.1080/j.1365-280X.1999.00222.x