دورية
In situ hybridization in paracoccidioidomycosis
العنوان: | In situ hybridization in paracoccidioidomycosis |
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المؤلفون: | De Brito, T., Sandhu, G. S., Kline, B. C., Aleff, R. A., Sandoval, M. P., Santos, R. T., Brandão, A. A. H., Lacaz, C. S. |
المصدر: | Medical Mycology; January 1999, Vol. 37 Issue: 3 p207-207, 1p |
مستخلص: | In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level. |
قاعدة البيانات: | Supplemental Index |
تدمد: | 13693786 14602709 |
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DOI: | 10.1080/j.1365-280X.1999.00222.x |