دورية
Total DNA Preparation Excluding Extracellular Acidic Polysaccharide from LipomycesYeasts and its Application to Taxonomic Studies
| العنوان: | Total DNA Preparation Excluding Extracellular Acidic Polysaccharide from LipomycesYeasts and its Application to Taxonomic Studies |
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| المؤلفون: | NISHIMURA, Katsushi, SHIMADA, Koji, IWASAWA, Hiroki, NAGANUMA, Takafumi, UZUKA, Yasuyuki |
| المصدر: | Bioscience, Biotechnology, and Biochemistry; January 2002, Vol. 66 Issue: 7 p1563-1566, 4p |
| مستخلص: | A preparation method of total DNA from Lipomycesyeasts was improved in order to exclude extracellular acidic polysaccharide thoroughly. The method combined an ultracentrifuge and polyethylene glycol precipitation with the usual method. The total DNAs obtained were analyzed for G+C content and by DNA-DNA hybridization. The results all agreed almost completely with literature data. All the DNA samples prepared using this method were pure enough for these taxonomic analyses and could also be used as templates of PCR for amplification of small subunit ribosomal DNA and the internal transcribed spacer region. |
| قاعدة البيانات: | Supplemental Index |
PDF full text from Publisher | تدمد: | 09168451 13476947 |
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| DOI: | 10.1271/bbb.66.1563 |