دورية
Antimalarial Activity of Granzyme B and Its Targeted Delivery by a Granzyme B–Single-Chain Fv Fusion Protein
| العنوان: | Antimalarial Activity of Granzyme B and Its Targeted Delivery by a Granzyme B–Single-Chain Fv Fusion Protein |
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| المؤلفون: | Kapelski, Stephanie, de Almeida, Melanie, Fischer, Rainer, Barth, Stefan, Fendel, Rolf |
| المصدر: | Antimicrobial Agents and Chemotherapy; October 2014, Vol. 59 Issue: 1 p669-672, 4p |
| مستخلص: | ABSTRACTWe present here the first evidence that granzyme B acts against Plasmodium falciparum(50% inhibitory concentration [IC50], 1,590 nM; 95% confidence interval [95% CI], 1,197 to 2,112 nM). We created a novel antimalarial fusion protein consisting of granzyme B fused to a merozoite surface protein 4 (MSP4)-specific single-chain Fv protein (scFv), which targets the enzyme to infected erythrocytes, with up to an 8-fold reduction in the IC50(176 nM; 95% CI, 154 to 202 nM). This study confirms the therapeutic efficacies of recombinant antibody-mediated antimalarial immunotherapeutics based on granzyme B. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00664804 10986596 |
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| DOI: | 10.1128/AAC.04190-14 |