دورية
Study of the Growth Regulation of Preimplantation Mouse Embryos Using Concanavalin A1
| العنوان: | Study of the Growth Regulation of Preimplantation Mouse Embryos Using Concanavalin A1 |
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| المؤلفون: | Pienkowski, Marek |
| المصدر: | Experimental Biology and Medicine; February 1974, Vol. 145 Issue: 2 p464-469, 6p |
| مستخلص: | Unfertilized eggs and preimplantation mouse embryos had the same binding capacity for Concanavalin A. Concanavalin A in doses of 10 μg/ml agglutinated preimplantation embryos and the embryos derived from parthenogenetically stimulated eggs, but did not agglutinate unfertilized eggs. Unfertilized eggs became agglutinable with Concanavalin A in doses of 10 μg/ml after prolonged pretreatment with pronase or if the concentration of Concanavalin A was raised to 2000 μg/ml. The presence of 50 μg/ml of Concanavalin A in the medium inhibited development of preimplantation mouse embryos. Inhibition of growth was partially reversible after treatment of Concanavalin A-treated embryos with methyl-α-D-mannopyranose.We are grateful to Dr. Max M. Burger for helpful consultation, Dr. Christopher Graham for providing parthenogenetically stimulated eggs, and Mrs. Marguerite Solomon for excellent technical assistance. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 15353702 15353699 |
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| DOI: | 10.3181/00379727-145-37832 |