Multiple-fluorophore-specie detection with a tapered Fabry–Perot fluorescence spectrometer

التفاصيل البيبلوغرافية
العنوان: Multiple-fluorophore-specie detection with a tapered Fabry–Perot fluorescence spectrometer
المؤلفون: Wahl, Jeremy A., Van Delden, Jay S., Tiwari, Sandip
المصدر: Applied Optics; September 2005, Vol. 44 Issue: 25 p5190-5197, 8p
مستخلص: A novel fluorescence spectrometer and method for the simultaneous detection of multiple-fluorophore species in a no-moving-parts, instantaneous manner is described. In the reported embodiment of the instrument, a tapered Fabry–Perot filter is used to spatially encode the fluorescence spectrum from a multiple-dye-containing test sample. Using a pseudoinverse reconstruction algorithm, we spectrally decode the particle concentration for each dye specie in the test sample. Experimental results are reported along with a theoretical treatment of the method.
قاعدة البيانات: Supplemental Index