دورية
Altered neuronal network and rescue in a human MECP2duplication model
| العنوان: | Altered neuronal network and rescue in a human MECP2duplication model |
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| المؤلفون: | Nageshappa, S, Carromeu, C, Trujillo, C A, Mesci, P, Espuny-Camacho, I, Pasciuto, E, Vanderhaeghen, P, Verfaillie, C M, Raitano, S, Kumar, A, Carvalho, C M B, Bagni, C, Ramocki, M B, Araujo, B H S, Torres, L B, Lupski, J R, Van Esch, H, Muotri, A R |
| المصدر: | Molecular Psychiatry; February 2016, Vol. 21 Issue: 2 p178-188, 11p |
| مستخلص: | Increased dosage of methyl-CpG-binding protein-2 (MeCP2) results in a dramatic neurodevelopmental phenotype with onset at birth. We generated induced pluripotent stem cells (iPSCs) from patients with the MECP2duplication syndrome (MECP2dup), carrying different duplication sizes, to study the impact of increased MeCP2 dosage in human neurons. We show that cortical neurons derived from these different MECP2dup iPSC lines have increased synaptogenesis and dendritic complexity. In addition, using multi-electrodes arrays, we show that neuronal network synchronization was altered in MECP2dup-derived neurons. Given MeCP2 functions at the epigenetic level, we tested whether these alterations were reversible using a library of compounds with defined activity on epigenetic pathways. One histone deacetylase inhibitor, NCH-51, was validated as a potential clinical candidate. Interestingly, this compound has never been considered before as a therapeutic alternative for neurological disorders. Our model recapitulates early stages of the human MECP2duplication syndrome and represents a promising cellular tool to facilitate therapeutic drug screening for severe neurodevelopmental disorders. |
| قاعدة البيانات: | Supplemental Index |
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Full Text Finder | تدمد: | 13594184 14765578 |
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| DOI: | 10.1038/mp.2015.128 |