دورية
Candida albicansAirway Colonization Facilitates Subsequent Acinetobacter baumanniiPneumonia in a Rat Model
| العنوان: | Candida albicansAirway Colonization Facilitates Subsequent Acinetobacter baumanniiPneumonia in a Rat Model |
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| المؤلفون: | Tan, Xiaojiang, Chen, Ruilan, Zhu, Song, Wang, Huijun, Yan, Dongxing, Zhang, Xiangdong, Farmakiotis, Dimitrios, Mylonakis, Eleftherios |
| المصدر: | Antimicrobial Agents and Chemotherapy; April 2016, Vol. 60 Issue: 6 p3348-3354, 7p |
| مستخلص: | ABSTRACTThe objective of the study was to determine the effects of Candida albicansrespiratory tract colonization on Acinetobacter baumanniipneumonia in a rat model. Rats were colonized with C. albicansby instillation of 3 × 106CFU into their airways, while sterile saline was instilled in the control group. The colonized rats were further divided into two groups: treated with amphotericin B or not. The rats were subsequently infected with A. baumannii(108CFU by tracheobronchial instillation). A. baumanniilung CFU counts, cytokine lung levels, and rates of A. baumanniipneumonia were compared between groups. In vitroexpression of A. baumanniivirulence genes was measured by reverse transcription (RT)-PCR after 24-hour incubation with C. albicansor with Mueller-Hinton (MH) broth alone. Rats with Candidacolonization developed A. baumanniipneumonia more frequently and had higher A. baumanniiCFU burdens and heavier lungs than controls. After A. baumanniiinfection, lung interleukin 17 (IL-17) concentrations were lower and gamma interferon (IFN-γ) concentrations were higher in Candida-colonized rats than in controls. Candida-colonized rats treated with amphotericin B had a decreased rate of A. baumanniipneumonia and lower IFN-γ levels but higher IL-17 levels than untreated rats. Expression of basC, barB, bauA, ptk, plc2, and pld2was induced while expression of ompAand abaIwas suppressed in A. baumanniicultured in the presence of C. albicans. C. albicanscolonization facilitated the development of A. baumanniipneumonia in a rat model. Among Candida-colonized rats, antifungal treatment lowered the incidence of A. baumanniipneumonia. These findings could be due to modification of the host immune response and/or expression of A. baumanniivirulence genes by Candidaspp. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00664804 10986596 |
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| DOI: | 10.1128/AAC.02180-15 |