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Simultaneous determination of metabolites of trimethylbenzenes, dimethylbenzylmercapturicacid and dimethylhippuric acid, in human urine by solid-phase extraction followed by liquid chromatography tandem mass spectrometry

التفاصيل البيبلوغرافية
العنوان: Simultaneous determination of metabolites of trimethylbenzenes, dimethylbenzylmercapturicacid and dimethylhippuric acid, in human urine by solid-phase extraction followed by liquid chromatography tandem mass spectrometry
المؤلفون: Moriwaki, Hiroshi, Watanabe, Ayumi, Arakawa, Ryuichi, Tsujimoto, Yuji, Shimizu, Mitsuru, Noda, Tsutomu, Warashina, Munehiro, Tanaka, Masanobu
المصدر: Journal of Mass Spectrometry; November 2002, Vol. 37 Issue: 11 p1152-1157, 6p
مستخلص: We describe a novel method for the determination of three kinds of dimethylbenzylmercapturic acids (DMM) and six kinds of dimethylhippuric acids (DMH), found in urine as metabolites of trimethylbenzenes, based on liquid chromatography/electrospray ionization tandem mass spectrometry. A solid-phase extraction procedure was used for the extractions of DMM and DMH from a urine sample, and the separation was performed on a reversed–phase C30 column. The analytes were ionized by electrospray in the positive-ion mode. Operating in the multiple reaction monitoring mode, the linearity of the relative mass spectrometric responses to the internal standard versus analyte concentrations were established in the range 0.1–100 ng ml−1. The extraction procedure was rapid and the relative standard deviations were below 5%. The detection limits of DMM and DMH in the urine by the proposed method were in the ranges 0.26–0.41 and 0.42–2.0 ng l−1, respectively. Furthermore, DMM and DMH were detected in a urine sample from an individual who did not suffer from occupational exposure to trimethylbenzenes, by using this method. Copyright © 2002 John Wiley & Sons, Ltd.
قاعدة البيانات: Supplemental Index
الوصف
تدمد:10765174
10969888
DOI:10.1002/jms.378