دورية
Characterization of miRNA Expression in Human Lumbar Disks
| العنوان: | Characterization of miRNA Expression in Human Lumbar Disks |
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| المؤلفون: | Ohrt-Nissen, S., Dahl, B., Friis-Hansen, L., Nielsen, F. C., Vikeså, J., Lajer, C., Rossing, M., Døssing, K. |
| المصدر: | Global Spine Journal; June 2012, Vol. 2 Issue: Supplement 1 ps-0032-131-s-0032-131 |
| مستخلص: | Introduction MicroRNA (miRNA) are short ∼22 nucleotide RNA sequences that regulate mRNA translation, and more than 900 humans miRNAs are known. miRNAs have been shown to play a role in synthesis of inflammatory mediators. Since inflammation play a role in intervertebral disk degeneration, we wanted to isolate miRNA from human lumbar intervertebral disks and subsequently characterize the difference in miRNA expression between the annulus fibrosus (AF) and nucleus pulposus.Materials and Methods A total of 14 patients undergoing anterior interbody fusion for degenerative disk disease of the lumbar spine were included. During surgery, biopsies from the intervertebral disks (IVD) were immediately stored in RNA-Later, transported to the laboratory within a day, and then frozen at −80°C. RNA extraction was performed using the Trizol method. Global miRNA expression analysis was performed using the Affymetrix GeneChip(r) miRNA array.Results We developed a method allowing for the extraction of miRNA from human IVDs, yielding 1 to 4 microgram of total RNA Per 100 mg of disk. Total 27 miRNAs had a higher expression in the fibrosus and 10 had the highest expression in the pulposus. Among the top 15 signaling pathways most likely to be controlled by these miRNA's were the TGFβ, PDGF, IGF EGF, and actin cytoskeletal pathway.Conclusion This is the first time miRNA have been demonstrated in the human IVD. The miRNA expression differs between the miRNA expressed in the nucleus pulposus and AF. The identified miRNAs control signaling pathways is important for maintenance of the IVD. Future studies may determine the importance of miRNA in the development of IVD disease. I confirm having declared any potential conflict of interest for all authors listed on this abstract No Disclosure of Interest None declared |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 21925682 21925690 |
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| DOI: | 10.1055/s-0032-1319947 |