دورية
Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Crerecombinase exclusively in eosinophils
| العنوان: | Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Crerecombinase exclusively in eosinophils |
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| المؤلفون: | Doyle, Alfred D., Jacobsen, Elizabeth A., Ochkur, Sergei I., Willetts, Lian, Shim, Kelly, Neely, Joseph, Kloeber, Jake, LeSuer, Will E., Pero, Ralph S., Lacy, Paige, Moqbel, Redwan, Lee, Nancy A., Lee, James J. |
| المصدر: | Journal of Leukocyte Biology; July 2013, Vol. 94 Issue: 1 p17-24, 8p |
| مستخلص: | Cre‐recombinase expression in eoCREmice generates a model system facilitating eosinophil‐specific knockouts, and/or, ectopic overexpression of heterologous genes. Eosinophils are generally linked to innate host defense against helminths, as well as the pathologies associated with allergic diseases, such as asthma. Nonetheless, the activities of eosinophils remain poorly understood, which in turn, has prevented detailed definitions of their role(s) in health and disease. Homologous recombination in embryonic stem cells was used to insert a mammalianized Crerecombinase in the ORF encoding Epx. This knock‐in strategy overcame previous inefficiencies associated with eosinophil‐specific transgenic approaches and led to the development of a knock‐in strain of mice (eoCRE), capable of mediating recombination of “floxed” reporter cassettes in >95% of peripheral blood eosinophils. We also showed that this Creexpression was limited exclusively to eosinophil‐lineage committed cells with no evidence of Cre‐mediated toxicity. The efficiency and specificity of Creexpression in eoCREmice were demonstrated further in a cross with a knock‐in mouse containing a “(flox‐stop‐flox)” DTA cassette at the ROSA26 locus, generating yet another novel, eosinophil‐less strain of mice. The development of eoCREmice represents a milestone in studies of eosinophil biology, permitting eosinophil‐specific gene targeting and overexpression in the mouse as part of next‐generation studies attempting to define eosinophil effector functions. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 07415400 19383673 |
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| DOI: | 10.1189/jlb.0213089 |