دورية
Monitoring H2O2inside Aspergillus fumigatuswith an Integrated Microelectrode: The Role of Peroxiredoxin Protein Prx1
| العنوان: | Monitoring H2O2inside Aspergillus fumigatuswith an Integrated Microelectrode: The Role of Peroxiredoxin Protein Prx1 |
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| المؤلفون: | Santos, Carla Santana, Bannitz-Fernandes, Renata, Lima, Alex S., Tairum, Carlos A., Malavazi, Iran, Netto, Luis E. S., Bertotti, Mauro |
| المصدر: | Analytical Chemistry; 20240101, Issue: Preprints |
| مستخلص: | Peroxiredoxins (Prx) are important proteins involved in hydroperoxide degradation and are related to virulence in several pathogens, including Aspergillus fumigatus. In this work, in vivo studies on the degradation of hydrogen peroxide (H2O2) in the microenvironment of A. fumigatusfungus were performed by using an integrated Pt microelectrode. Three A. fumigatusstrains were used to confirm the role of the cytosolic protein Prx1 in the defense mechanism of this microorganism: a wild-type strain, capable to expressing the protein Prx1; a Δprxstrain, whose gene prx1was removed; and a genetically complemented Δprx1::prx1+strain generated from the Δprx1and in which the gene prx1was reintroduced. The fabricated microelectrode was shown to be a reliable inert probe tip for in situ and real-time measurements of H2O2in such microenvironments, with potential applications in investigations involving the mechanism of oxidative stress. |
| قاعدة البيانات: | Supplemental Index |
Find this issue from the American Chemical Society | تدمد: | 00032700 15206882 |
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| DOI: | 10.1021/acs.analchem.7b04074 |