دورية
Effects of combined menaquinone-4 and PTH1–34treatment on osetogenesis and angiogenesis in calvarial defect in osteopenic rats
| العنوان: | Effects of combined menaquinone-4 and PTH1–34treatment on osetogenesis and angiogenesis in calvarial defect in osteopenic rats |
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| المؤلفون: | Weng, She-Ji, Xie, Zhong-Jie, Wu, Zong-Yi, Yan, De-Yi, Tang, Jia-Hao, Shen, Zi-Jian, Li, Hang, Bai, Bing-Li, Boodhun, Viraj, (Eric) Dong, Xiang, Yang, Lei |
| المصدر: | Endocrine; February 2019, Vol. 63 Issue: 2 p376-384, 9p |
| مستخلص: | The aim of this study was to evaluate the effect of combining human parathyroid hormone (1–34) (PTH1–34; PTH) and menaquinone-4 (MK-4) on calvarial bone defect repair in osteopenic rats. Fourteen week olds were subject to craniotomy for the establishment of osteopenic animal models fed through a chronically low-protein diet. After that, critical calvarial defect model was established and all rats were randomly divided into four groups: sham, MK-4, PTH, and PTH + MK-4. The animals received MK-4 (30 mg/kg/day), PTH1–34(60 μg/kg, three times a week), or PTH1–34(60 μg/kg, three times a week) plus MK-4 (30 mg/kg/day) for 8 weeks, respectively. Serum γ-carboxylated osteocalcin (Gla-OC) levels, histological and immunofluorescent labeling were employed to evaluate the bone formation and mineralization in calvarial bone defect. In addition, Microfil perfusion, immunohistochemical, and micro-CT suggested enhanced angiogenesis and bone formation in calvarial bone healing. In this study, treatment with either PTH1–34or MK-4 promoted bone formation and vascular formation in calvarial bone defects compared with the sham group. In addition, combined treatment of PTH1–34plus MK-4 increased serum level of Gla-OC, improved vascular number and vascular density, and enhanced bone formation in calvarial bone defect in osteopenic conditions as compared with monotherapy. In summary, this study indicated that PTH1–34plus MK-4 combination therapy accelerated bone formation and angiogenesis in calvarial bone defects in presence of osteopenia. |
| قاعدة البيانات: | Supplemental Index |
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Full Text Finder | تدمد: | 1355008x 15590100 |
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| DOI: | 10.1007/s12020-018-1761-7 |