دورية
Targeting of a Tropomyosin Isoform to Short Microfilaments Associated with the Golgi Complex
| العنوان: | Targeting of a Tropomyosin Isoform to Short Microfilaments Associated with the Golgi Complex |
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| المؤلفون: | Percival, Justin M., Hughes, Julie A. I., Brown, Darren L., Schevzov, Galina, Heimann, Kirsten, Vrhovski, Bernadette, Bryce, Nicole, Stow, Jennifer L., Gunning, Peter W. |
| المصدر: | Molecular Biology of the Cell; January 2004, Vol. 15 Issue: 1 p268-280, 13p |
| مستخلص: | A growing body of evidence suggests that the Golgi complex contains an actin-based filament system. We have previously reported that one or more isoforms from the tropomyosin gene Tm5NM (also known as γ-Tm), but not from either the α- or β-Tm genes, are associated with Golgi-derived vesicles (Heimann et al.,(1999). J. Biol. Chem. 274,10743-10750). We now show that Tm5NM-2 is sorted specifically to the Golgi complex, whereas Tm5NM-1, which differs by a single alternatively spliced internal exon, is incorporated into stress fibers. Tm5NM-2 is localized to the Golgi complex consistently throughout the G1 phase of the cell cycle and it associates with Golgi membranes in a brefeldin A-sensitive and cytochalasin D-resistant manner. An actin antibody, which preferentially reacts with the ends of microfilaments, newly reveals a population of short actin filaments associated with the Golgi complex and particularly with Golgi-derived vesicles. Tm5NM-2 is also found on these short microfilaments. We conclude that an alternative splice choice can restrict the sorting of a tropomyosin isoform to short actin filaments associated with Golgi-derived vesicles. Our evidence points to a role for these Golgi-associated microfilaments in vesicle budding at the level of the Golgi complex. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 10591524 19394586 |
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| DOI: | 10.1091/mbc.e03-03-0176 |