دورية
Production of a specific polyclonal antibody against the rat β thyroid receptor, using synthetic peptide as antigen
| العنوان: | Production of a specific polyclonal antibody against the rat β thyroid receptor, using synthetic peptide as antigen |
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| المؤلفون: | Puymirat, J., Gadbois, P., Dussault, L., Garceau, L., Dussault, J. H. |
| المصدر: | European Journal of Endocrinology; October 1991, Vol. 125 Issue: 4 p397-400, 4p |
| مستخلص: | We have developed a polyclonal antibody to a synthetic peptide corresponding to the deduced amino acid residues 67–80 of the amino terminal sequence of the rat thyroid receptor β cDNA. Immunopositive sera were identifed by radioimmunoassay and purified by affinity chromatography. The antibody specifically immunoprecipitated the labelled thyroid receptor β synthetized in vitro but not the rat thyroid receptor α. Furthermore, 38% of the L-T3binding capacity of the rat liver nuclear receptor were immunoabsorbed by this antibody. By immunocytochemistry, this antibody stained specifically the nuclei of hepatocytes while no staining was observed in the rat testis. This study presents a specific polyclonal antibody against the thyroid receptor β. This antibody will be of a great help in determining the role of the β receptors in the mechanism of action of thyroid hormone. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 08044643 1479683X |
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| DOI: | 10.1530/acta.0.1250397 |