التفاصيل البيبلوغرافية
| العنوان: |
Detection of foot-and-mouth disease antigen in bovine epithelial samples: comparison of sites of sample collection by an enzyme linked immunosorbent assay (elisa) and complement fixation test |
| المؤلفون: |
OLIVER, R.E., DONALDSON, A.I., GIBSON, C.F., ROEDER, P.L., LE BLANC SMITH, P.M., HAMBLIN, C. |
| المصدر: |
Research in Veterinary Science; May 1988, Vol. 44 Issue: 3 p315-319, 5p |
| مستخلص: |
Serially collected epithelial samples from lesions in the mouth and on the feet of calves experimentally infected with foot-and-mouth disease (fmd) type O1bfs1860 were assayed for the presence of fmdviral antigen using a double antibody sandwich enzyme-linked immunosorbent assay (elisa) and a complement fixation (cf) test. The amount of infectious virus in each sample was also determined. fmdviral antigen was detected by elisain 70 per cent of the mouth samples and 92 per cent of samples from the feet. The cftest was less sensitive; it detected antigen in 44 per cent of mouth and 85 per cent of foot samples. In mouth samples the amount of antigen decreased rapidly becoming undetectable by the fourth day of sampling whereas in foot samples the quantity of antigen declined more slowly, and could be detected until the seventh day of sampling. Therefore it was concluded that the age of lesion and the site from which epithelial samples are collected are both important determinants in the laboratory diagnosis of fmd. In cattle, foot lesions are more likely than mouth lesions to yield antigen and to remain positive for a longer period. |
| قاعدة البيانات: |
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