دورية
Proteomic Delineation of the ArcA Regulon in SalmonellaTyphimurium During Anaerobiosis*
| العنوان: | Proteomic Delineation of the ArcA Regulon in SalmonellaTyphimurium During Anaerobiosis* |
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| المؤلفون: | Wang, Zhen, Sun, Jingjing, Xia, Tingying, Liu, Yanhua, Fu, Jiaqi, Lo, Yat Kei, Chang, Cheng, Yan, Aixin, Liu, Xiaoyun |
| المصدر: | Molecular and Cellular Proteomics (MCP Online); October 2018, Vol. 17 Issue: 10 p1937-1947, 11p |
| مستخلص: | Salmonella entericaserovar Typhimurium (S.Typhimurium) is one of the most used models for bacterial pathogenesis and successful infection requires its adaptation to the low oxygen environment in host gastrointestinal tracts. Central to this process is the Arc (aerobic respiratory control) two-component regulatory system that contains a sensor kinase ArcB and a response regulator ArcA. Nevertheless, a comprehensive profile of the ArcA regulon on the proteome level is still lacking in S.Typhimurium. Here we quantitatively profiled Salmonellaproteome during anaerobiosis in an arcA-deleting mutant compared with its parental strain. In addition to known processes under its control, notably we found that ArcA represses ethanolamine utilization by directly binding to the promoter region of the eutoperon. Furthermore, we found opposing changes of several bacterial genes on the protein and transcript levels in the arcA-deleting mutant including the virulence genes of Salmonellapathogenicity island 1 (SPI-1), thereby indicating potentially prevalent post-transcriptional regulatory mechanisms. Altogether, our study provides important new insights into ArcA-dependent bacterial physiology and virulence during Salmonellaanaerobiosis. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 15359476 15359484 |
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| DOI: | 10.1074/mcp.RA117.000563 |