دورية
Thyroid hormone modulates androgen and oestrogen receptor content in the Sertoli cells of peripubertal rats
| العنوان: | Thyroid hormone modulates androgen and oestrogen receptor content in the Sertoli cells of peripubertal rats |
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| المؤلفون: | Panno, M L, Sisci, D, Salerno, M, Lanzino, M, Pezzi, V, Morrone, E G, Mauro, L, Palmero, S, Fugassa, E, Andò, S |
| المصدر: | Journal of Endocrinology; January 1996, Vol. 148 Issue: 1 p43-50, 8p |
| مستخلص: | A possible role of tri-iodothyronine (T3) on the interplay between testicular steroids and Sertoli cells has been investigated on the basis of previous findings demonstrating a direct inhibitory influence of T3on aromatase activity and oestradiol production in peripuberal Sertoli cells. In this context, the present study was focused on the effects of T3on oestrogen receptor (ER) and androgen receptor (AR) contents in the cytosol and nucleus of Sertoli cells isolated from 2-, 3- and 4-week-old euthyroid, hypothyroid and hypothyroid treated rats.Hypothyroidism was induced by the oral administration of 0·025% methimazole (MMI) from birth until the rats were killed at 2, 3 and 4 weeks of age. Half of the MMI-treated animals were injected i.p. with l-tri-iodothyronine (T3; 3 μg/100 g body weight) during the last week before death. Sertoli cells from all groups were initially cultured under basal conditions for the first 24 h and subsequently in the presence of testosterone with or without T3for an additional 24 h. Hypothyroidism was associated with severe impairment of body as well as testicular growth. Euthyroid ERs showed an elevated Kd(0·76 nm) which was similar in the different age groups investigated. The in vitroaddition of T3or testosterone induced a decrease in ER content and this decrease was greater after exposure to both hormones. In 2- and 3-week-old hypothyroid rats, ER content was markedly increased and was reversed in euthyroid rats when T3was given in vivo. When ERs were assayed in the Sertoli cell nucleus and cytoplasm of 2- and 3-week-old animals, a strong relationship in ER content in the two cellular compartments was observed. Neither of the hormones tested seemed to affect the AR content in the nucleus significantly, while the in vitroaddition of testosterone or T3or both hormones together augmented the ARs in the cytosol to a greater extent, resulting in an increase in their total (cytosolic and nuclear) content in the cells.The present data suggest that T3down-regulates ERs and up-regulates ARs in peripuberal Sertoli cells. The additive effect of testosterone and T3in up-regulating ARs could possibly involve a role for T3in influencing the androgen responsiveness of the Sertoli cells during spermatogenesis.Journal of Endocrinology(1996) 148,43–50 |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00220795 14796805 |
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| DOI: | 10.1677/joe.0.1480043 |