دورية
Expression of IGF-I and IGF-binding protein genes in cirrhotic liver
| العنوان: | Expression of IGF-I and IGF-binding protein genes in cirrhotic liver |
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| المؤلفون: | Ross, R J M, Chew, S L, D'Souza Li, L, Yateman, M, Rodriguez-Arnao, J, Gimson, A, Holly, J, Camacho-Hubner, C |
| المصدر: | Journal of Endocrinology; May 1996, Vol. 149 Issue: 2 p209-216, 8p |
| مستخلص: | The liver plays a central role in the IGF-I axis producing the majority of circulating hormone and some of its binding proteins (IGFBPs). Cirrhosis of the liver is characterised by changes in IGF-I and IGFBPs associated with liver fibrosis and regeneration. We have studied steady state levels of mRNA for the genes in the IGF-I axis in normal and cirrhotic human liver, localised the most highly expressed gene, IGFBP-1, and measured circulating IGFBP-3 by radioimmunoassay (RIA), IGFBP-2 and IGFBP-3 by Western ligand blot (WLB), and protease activity for IGFBP-3 in cirrhotic patients. Messenger RNA for IGF-I, IGFBP-1, IGFBP-2, and IGFBP-3 was detectable by Northern blotting in normal and cirrhotic liver although there was considerable variation in expression. IGFBP-2 and IGFBP-3 tended to be more highly expressed in cirrhotic liver and IGFBP-1 was more highly expressed in normal liver, although there were no significant differences. In normal liver, in situhybridisation localised IGFBP-1 to hepatocytes. In cirrhotic liver the regenerating nodules showed expression of IGFBP-1 while there was none in fibrotic tissue. Circulating IGFBP-3 levels were low as measured by RIA and WLB but protease activity was only found in one patient. IGFBP-2 levels, assessed by WLB, were similar to the normal serum pool. Our data show that key mRNAs involved in the IGF-I axis continue to be expressed in cirrhotic liver despite end stage liver disease. The low levels of IGFBP-3 do not appear to be due to reduced gene transcription or increased protease activity.Journal of Endocrinology(1996) 149,209–216 |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00220795 14796805 |
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| DOI: | 10.1677/joe.0.1490209 |