دورية
Studies of cisplatin and hemoglobin interactions using nanospray mass spectrometry and liquid chromatography with inductively-coupled plasma mass spectrometry
| العنوان: | Studies of cisplatin and hemoglobin interactions using nanospray mass spectrometry and liquid chromatography with inductively-coupled plasma mass spectrometry |
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| المؤلفون: | Mandal, Rupasri, Teixeira, Christine, Li, Xing-Fang |
| المصدر: | Analyst; 2003, Vol. 128 Issue: 6 p629-634, 6p |
| مستخلص: | Interactions of cisplatin with hemoglobin (Hb) were studied using both nanoelectrospray mass spectrometry (nanoESI-MS) and a combination of size exclusion high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICPMS). Size exclusion HPLC separation of free and protein-bound cisplatin followed by simultaneous monitoring of 195Pt and 57Fe demonstrated the presence of Hb-bound Pt complexes. Nanospray quadrupole time-of-flight mass spectrometry studies of the Hbcisplatin complexes further demonstrated the specific binding of cisplatin to the α-chain, heme-α, β-chain, and heme-β units of hemoglobin. Accurate mass measurements and tandem mass spectrometry information confirmed the Hbcisplatin complexes. The formation of Hbcisplatin complexes was observed at the sub-µM to µM concentration levels of cisplatin, which are relevant to clinical levels. These findings and the techniques developed for cisplatinHb interaction studies are useful for understanding of drugprotein interactions. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00032654 13645528 |
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