دورية
Composition of Antigen-Precipitin Precipitate.
| العنوان: | Composition of Antigen-Precipitin Precipitate. |
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| المؤلفون: | Wu, Hsien, Sah, Peter P. T., Li, C. P. |
| المصدر: | Experimental Biology and Medicine; June 1929, Vol. 26 Issue: 9 p737-738, 2p |
| مستخلص: | In a previous study1on the composition of the precipitate produced by the reaction of an antigen with its anti-serum, we used hemoglobin as the antigen, because we had a specific method for the determination of small amounts of this protein.On account of the difficulty of preparing anti-hemoglobin serum, hemoglobin is not a suitable antigen for the further study of the problem. We have, therefore, looked for some other protein which is a stronger antigen than hemoglobin and which also lends itself to quantitative determination in a mixture of proteins. Iodo-albumin has been found to serve our purpose, and we will report some results which have been obtained with it.The iodo-albumin is prepared as follows: 100 cc. of 5% sodium carbonate was added to 400 cc. of 2.5% crystallized egg-albumin solution. After cooling to 0°, the solution was mixed with an iodine solution prepared by dissolving 5 g. of iodine in 150 cc. 10% KI solution. The mixture was allowed to stand in the ice-box for 24 hours. The iodo-albumin formed was precipitated by neutralizing the solution and then adding a slight excess of acetic acid. The precipitate was washed twice with water by centrifuging. It was then dissolved in 200 cc. N/10 Na2CO3to give an approximately 1% solution. It is free from iodide and iodine and has a yellow color. The iodo-albumin prepared in this way contained 14.1% nitrogen and 6.9% iodine.Iodo-albumin is a good antigen. No difficulty was encountered in preparing the anti-serum. Rabbits were immunized by giving 3 series of injections. In each series, intravenous injection of 5 to 10 cc. of 1% iodo-albumin was given daily for 3 days. Rabbits were then rested for 3 days before another series was started. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 15353702 15353699 |
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| DOI: | 10.3181/00379727-26-4488 |