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A rickettsial isolate was obtained from a partially engorged Ixodes pacificusfemale, which was collected from Humboldt County, California. The isolate was provisionally named Rickettsiaendosymbiont Ixodes pacificus(REIP). The REIP isolate displayed the highest nucleotide sequence identity to Rickettsiaspecies phylotype G021 in I. pacificus(99%, 99%, and 100% for ompA, 16S rRNA, and gltA, respectively), a bacterium that was previously identified in I. pacifiucsby PCR. Analysis of sequences from complete opening frames of five genes, 16S rRNA, gltA, ompA, ompB,and sca4, provided inference to the bacteria’s classification among other Rickettsiaspecies. The REIP isolate displayed 99.8%, 99.4%, 99.2%, 99.5%, and 99.6% nucleotide sequence identity for 16S rRNA, gltA, ompA, ompB,and sca4gene, respectively, with genes of ‘R. monacensis’ str. IrR/Munich, indicating the REIP isolate is closely related to ‘R. monacensis’. Our suggestion was further supported by phylogenetic analysis using concatenated sequences of 16S rRNA, gltA, ompA, ompB,and sca4genes, concatenated sequences of dksA-xerC, mppA-purC, and rpmE-tRNAfMetintergenic spacer regions. Both phylogenetic trees implied that the REIP isolate is most closely related to ‘R. monacensis’str. IrR/Munich. We propose the bacterium be considered as ‘Rickettsia monacensis’ str. Humboldt for its closest phylogenetic relative (=DSM 103975 T = ATCC TSD-94 T). |
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