التفاصيل البيبلوغرافية
| العنوان: |
Resolving MiSeq-Generated Ambiguities in HLA-DPB1Typing by Using the Oxford Nanopore Technology |
| المؤلفون: |
Duke, Jamie L., Mosbruger, Timothy L., Ferriola, Deborah, Chitnis, Nilesh, Hu, Taishan, Tairis, Nikolaos, Margolis, David J., Monos, Dimitri S. |
| المصدر: |
The Journal of Molecular Diagnostics; September 2019, Vol. 21 Issue: 5 p852-861, 10p |
| مستخلص: |
The technical limitations of current next-generation sequencing technologies, combined with an ever-increasing number of human leukocyte antigen (HLA) alleles, form the basis for the additional ambiguities encountered at an increasing rate in clinical practice. HLA-DPB1characterization, particularly, generates a significant percentage of ambiguities (25.5%), posing a challenge for accurate and unambiguous HLA-DPB1genotyping. Phasing of exonic heterozygous positions between exon 2 and all other downstream exons has been the major cause of ambiguities. In this study, the Oxford Nanopore MinION, a third-generation sequencing technology, was used to resolve the phasing. The accurate MiSeq sequencing data, combined with the long reads obtained from the MinION platform, allow for the resolution of the tested ambiguities. |
| قاعدة البيانات: |
Supplemental Index |
Full Text via ClinicalKey 
Full Text Finder